Modulation of Mg2+ influx and cytoplasmic free Mg2+ concentration in rat ventricular myocytes
نویسندگان
چکیده
منابع مشابه
Cytoplasmic pH and free Mg2+ in lymphocytes
Measurements have been made of cytoplasmic pH, (pHi) and free Mg2+ concentration, ( [Mg2+]i), in pig and mouse lymphocytes. pHi was measured in four ways: by a digitonin null-point technique; by direct measurement of the pH of freeze-thawed cell pellets; from the 31P nuclear magnetic resonance (NMR) spectrum of intracellular inorganic phosphate; and by the use of a newly synthesized, intracellu...
متن کاملEffects of membrane potential on Na+ -dependent Mg2+ extrusion from rat ventricular myocytes.
To study Mg2+ transport across the cell membrane, the cytoplasmic concentration of Mg2+ ([Mg2+](i)) in rat ventricular myocytes was measured with the fluorescent indicator furaptra (mag-fura-2) under Ca2+ -free conditions (0.1 mM EGTA) at 25 degrees C. The fluorescence ratio signal of furaptra was converted to [Mg2+](i) using calibration parameters previously estimated in myocytes (Watanabe and...
متن کاملConcentration, compartmentation and metabolic function of intracellular free Mg2+.
Intracellular total Mg2+ and free Mg2+ are compartmentalized between cell organelles and within the cytosol. Different values of [Mg2+]i in the cytosol of the same cell type were measured by various investigators. A main reason for the differences is the uncertainty of the dissociation constants used for the Mg furaptra complex in the fluorescence method and for MgATP when 31P NMR was employed....
متن کاملModulation of the Local SR Ca2+ Release by Intracellular Mg2+ in Cardiac Myocytes
In cardiac muscle, Ca(2+)-induced Ca(2+) release (CICR) from the sarcoplasmic reticulum (SR) defines the amplitude and time course of the Ca(2+) transient. The global elevation of the intracellular Ca(2+) concentration arises from the spatial and temporal summation of elementary Ca(2+) release events, Ca(2+) sparks. Ca(2+) sparks represent the concerted opening of a group of ryanodine receptors...
متن کاملCalcium influx through If channels in rat ventricular myocytes.
The hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels, or cardiac (I(f))/neuronal (I(h)) time- and voltage-dependent inward cation current channels, are conventionally considered as monovalent-selective channels. Recently we discovered that calcium ions can permeate through HCN4 and I(h) channels in neurons. This raises the possibility of Ca(2+) permeation in I(f), the I(h) co...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Physiological Sciences
سال: 2018
ISSN: 1880-6546,1880-6562
DOI: 10.1007/s12576-018-0625-5